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 | Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha : |
11/06/2018 |
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Actualizado : |
09/10/2019 |
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Tipo de producción científica : |
Trabajos en Congresos/Conferencias |
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Autor : |
ADDIEGO, A.; CARRIÓN, F.; OLIVERO, N.; PLA, M.; RIET-CORREA, F.; BIANCHI, S.; PRITSCH, O. |
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Afiliación : |
ANDRÉS ADDIEGO, Laboratorio de Inmunovirología,Instituto Pasteur Montevideo, Uruguay.; FEDERICO CARRIÓN, Laboratorio de Inmunovirología,Instituto Pasteur Montevideo, Uruguay.; NATALIA OLIVERO, Laboratorio de Inmunovirología,Instituto Pasteur Montevideo, Uruguay.; MARCELO PLA TEJERA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FRANKLIN RIET-CORREA AMARAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; SERGIO BIANCHI, Laboratorio de Inmunovirología,Instituto Pasteur Montevideo, Uruguay.; OTTO PRITSCH, Laboratorio de Inmunovirología,Instituto Pasteur Montevideo, Uruguay. |
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Título : |
Desarrollo de un inmunoensayo para el diagnóstico de la infección por el virus de la leucemia bovina y su utilización en el análisis evolutivo de la infección. |
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Fecha de publicación : |
2018 |
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Fuente / Imprenta : |
In: Jornadas Uruguayas de Buiatría, 46., 2018, Paysandú, Uy., Matto, C.; Delpiazzo, R.(Ed.). Trabajos presentados. Paysandú: Centro Médico Veterinario de Paysandú/Sociedad Uruguaya de Buiatría, 2018. |
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Páginas : |
p.189-191. |
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Idioma : |
Español |
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Palabras claves : |
LEUCOSIS BOVINA ENZOÓTICA (LBE); PLATAFORMA SALUD ANIMAL; VIRUS DE LA LEUCEMIA BOVINA (VLB). |
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Thesagro : |
ENFERMEDADES INFECCIOSAS. |
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Asunto categoría : |
L73 Enfermedades de los animales |
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Marc : |
LEADER 00950naa a2200241 a 4500
001 1058698
005 2019-10-09
008 2018 bl uuuu u00u1 u #d
100 1 $aADDIEGO, A.
245 $aDesarrollo de un inmunoensayo para el diagnóstico de la infección por el virus de la leucemia bovina y su utilización en el análisis evolutivo de la infección.
260 $c2018
300 $ap.189-191.
650 $aENFERMEDADES INFECCIOSAS
653 $aLEUCOSIS BOVINA ENZOÓTICA (LBE)
653 $aPLATAFORMA SALUD ANIMAL
653 $aVIRUS DE LA LEUCEMIA BOVINA (VLB)
700 1 $aCARRIÓN, F.
700 1 $aOLIVERO, N.
700 1 $aPLA, M.
700 1 $aRIET-CORREA, F.
700 1 $aBIANCHI, S.
700 1 $aPRITSCH, O.
773 $tIn: Jornadas Uruguayas de Buiatría, 46., 2018, Paysandú, Uy., Matto, C.; Delpiazzo, R.(Ed.). Trabajos presentados. Paysandú: Centro Médico Veterinario de Paysandú/Sociedad Uruguaya de Buiatría, 2018.
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INIA La Estanzuela (LE) |
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| Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
01/10/2014 |
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Actualizado : |
09/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
A - 1 |
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Autor : |
ALQUEZAR, B.; RODRIGO, M.J.; LADO, J.; ZACARÍAS, L. |
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Afiliación : |
JOANNA LADO LINDNER, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
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Título : |
A comparative physiological and transcriptional study of carotenoid biosynthesis in white and red grapefruit (Citrus paradisi Macf.). |
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Fecha de publicación : |
2013 |
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Fuente / Imprenta : |
Tree Genetics & Genomes, 2013, v.9, no.5, p.1257-1269. |
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ISSN : |
1614-2942 |
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DOI : |
10.1007/s11295-013-0635-7 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 30 January 2013 / Revised: 26 March 2013 / Accepted: 15 April 2013 / Published online: 15 May 2013. |
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Contenido : |
ABSTRACT.
Accumulation of lycopene in citrus fruits is an unusual feature restricted to selected mutants. Grapefruit (Citrus paradisi Macf.) is the Citrus specie with greater number of red-fleshed mutants, but the molecular bases of this alteration are not fully understood. To gain knowledge into the mechanisms implicated in this alteration, we conducted a comparative analysis of carotenoid profile and of the expression of genes related to carotenoid biosynthesis and catabolism in flavedo and pulp of two grapefruit cultivars with marked differences in colouration: the white Marsh and the red Star Ruby. Mature green fruit of Marsh accumulated chloroplastic carotenoids, while mature tissues lacked carotenoids. However, accumulation of downstream products such as abscisic acid (ABA) and expression of its biosynthetic genes, 9-cis-epoxycarotenoid dioxygenase (NCED1 and NCED2), increased after the onset of colouration. In contrast, red grapefruit accumulated lycopene, phytoene and phytofluene, while ABA content and NCED gene expression were lower than in Marsh, suggesting a blockage in the carotenoid biosynthetic pathway. Expression analysis of three genes of the isoprenoid pathway and nine of the carotenoid biosynthetic pathway revealed virtually no differences in flavedo and pulp between both genotypes, except for the chromoplast-specific lycopene cyclase 2 (β-LCY2) which was lower in the pulp of the red grapefruit. The proportion in the expression of the allele with high (β-LCY2a) and low (β-LCY2b) activity was also similar in the pulp of both genotypes. Therefore, results suggest that reduced expression of β-LCY2 appears to be responsible of lycopene accumulation in the red Star Ruby grapefruit.
© 2013 Springer-Verlag Berlin Heidelberg. MenosABSTRACT.
Accumulation of lycopene in citrus fruits is an unusual feature restricted to selected mutants. Grapefruit (Citrus paradisi Macf.) is the Citrus specie with greater number of red-fleshed mutants, but the molecular bases of this alteration are not fully understood. To gain knowledge into the mechanisms implicated in this alteration, we conducted a comparative analysis of carotenoid profile and of the expression of genes related to carotenoid biosynthesis and catabolism in flavedo and pulp of two grapefruit cultivars with marked differences in colouration: the white Marsh and the red Star Ruby. Mature green fruit of Marsh accumulated chloroplastic carotenoids, while mature tissues lacked carotenoids. However, accumulation of downstream products such as abscisic acid (ABA) and expression of its biosynthetic genes, 9-cis-epoxycarotenoid dioxygenase (NCED1 and NCED2), increased after the onset of colouration. In contrast, red grapefruit accumulated lycopene, phytoene and phytofluene, while ABA content and NCED gene expression were lower than in Marsh, suggesting a blockage in the carotenoid biosynthetic pathway. Expression analysis of three genes of the isoprenoid pathway and nine of the carotenoid biosynthetic pathway revealed virtually no differences in flavedo and pulp between both genotypes, except for the chromoplast-specific lycopene cyclase 2 (β-LCY2) which was lower in the pulp of the red grapefruit. The proportion in the expression of the allele with high... Presentar Todo |
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Palabras claves : |
ABA; CAROTENOIDS; GENE EXPRESSION; GRAPEFRUIT; LYCOPENE. |
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Thesagro : |
CAROTENO; CITRUS. |
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Asunto categoría : |
F01 Cultivo |
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Marc : |
LEADER 02702naa a2200277 a 4500
001 1050762
005 2019-10-09
008 2013 bl uuuu u00u1 u #d
022 $a1614-2942
024 7 $a10.1007/s11295-013-0635-7$2DOI
100 1 $aALQUEZAR, B.
245 $aA comparative physiological and transcriptional study of carotenoid biosynthesis in white and red grapefruit (Citrus paradisi Macf.).$h[electronic resource]
260 $c2013
500 $aArticle history: Received: 30 January 2013 / Revised: 26 March 2013 / Accepted: 15 April 2013 / Published online: 15 May 2013.
520 $aABSTRACT. Accumulation of lycopene in citrus fruits is an unusual feature restricted to selected mutants. Grapefruit (Citrus paradisi Macf.) is the Citrus specie with greater number of red-fleshed mutants, but the molecular bases of this alteration are not fully understood. To gain knowledge into the mechanisms implicated in this alteration, we conducted a comparative analysis of carotenoid profile and of the expression of genes related to carotenoid biosynthesis and catabolism in flavedo and pulp of two grapefruit cultivars with marked differences in colouration: the white Marsh and the red Star Ruby. Mature green fruit of Marsh accumulated chloroplastic carotenoids, while mature tissues lacked carotenoids. However, accumulation of downstream products such as abscisic acid (ABA) and expression of its biosynthetic genes, 9-cis-epoxycarotenoid dioxygenase (NCED1 and NCED2), increased after the onset of colouration. In contrast, red grapefruit accumulated lycopene, phytoene and phytofluene, while ABA content and NCED gene expression were lower than in Marsh, suggesting a blockage in the carotenoid biosynthetic pathway. Expression analysis of three genes of the isoprenoid pathway and nine of the carotenoid biosynthetic pathway revealed virtually no differences in flavedo and pulp between both genotypes, except for the chromoplast-specific lycopene cyclase 2 (β-LCY2) which was lower in the pulp of the red grapefruit. The proportion in the expression of the allele with high (β-LCY2a) and low (β-LCY2b) activity was also similar in the pulp of both genotypes. Therefore, results suggest that reduced expression of β-LCY2 appears to be responsible of lycopene accumulation in the red Star Ruby grapefruit. © 2013 Springer-Verlag Berlin Heidelberg.
650 $aCAROTENO
650 $aCITRUS
653 $aABA
653 $aCAROTENOIDS
653 $aGENE EXPRESSION
653 $aGRAPEFRUIT
653 $aLYCOPENE
700 1 $aRODRIGO, M.J.
700 1 $aLADO, J.
700 1 $aZACARÍAS, L.
773 $tTree Genetics & Genomes, 2013$gv.9, no.5, p.1257-1269.
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